1 November 2005 Genome-wide Examination of the Natural Solar Radiation Response in Shewanella oneidensis MR-1
Xiaoyun Qiu, James M. Tiedje, George W. Sundin
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We previously reported that Shewanella oneidensis MR-1 is extremely sensitive to natural solar radiation (NSR). Here we analyzed the global transcriptional profile of MR-1 during a 1-h recovering period after exposure to ambient solar light at a dose that yields about 20% survival rate on a Luria-Bertani (LB) plate. We observed the induction of DNA damage-repair genes, the SOS response as well as detoxification strategies that we previously observed in MR-1 following artificial UV-A irradiation. Few prophage-related genes were induced by natural solar UV radiation, however, in contrast to what was observed following artificial UV-B irradiation. Overall, the cellular response to NSR in MR-1 was more similar to that of UV-A than that of UV-B, but additional genes involved in detoxification were induced compared with induction by either UV-B or UV-A or their sum. Thus, oxidative stress appeared to contribute greatly to the NSR-induced cytotoxic effects in MR-1. A total of 29.1% of genome showed differential expression following NSR exposure, which is much greater than following exposure by UV-B (4.0%), UV-A (8.2%) or their sum (10.7%). Our data suggest that NSR may impact biological processes in a much more complex manner than previously thought.

Xiaoyun Qiu, James M. Tiedje, and George W. Sundin "Genome-wide Examination of the Natural Solar Radiation Response in Shewanella oneidensis MR-1," Photochemistry and Photobiology 81(6), 1559-1568, (1 November 2005). https://doi.org/10.1562/2005-04-15-RA-490
Received: 15 April 2005; Accepted: 1 July 2005; Published: 1 November 2005

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