This study analyzes the relationship between state-level variables and Twitter discourse on genetically modified organisms (GMOs). Using geographically identified tweets related to GMOs, we examined how the sentiments expressed about GMOs related to education levels, news coverage, proportion of rural and urban counties, state-level political ideology, amount of GMO-related legislation introduced, and agricultural dependence of each U.S. state. State-level characteristics predominantly did not predict the sentiment of the discourse. Instead, the topics of tweets predicted the majority of variance in tweet sentiment at the state level. The topics that tweets within a state focused on were related to state-level characteristics in some cases.