The Tabanidae are a diverse family of true flies whose members have both medical and veterinary importance. Tabanid species are known to occupy a variety of habitats worldwide, including the Great Smoky Mountains National Park, an area in the Nearctic Region known to contain considerable biodiversity. This report provides detailed information about previous collections made within the Park's boundaries and brings the number of currently known tabanid species residing in the Great Smoky Mountains National Park from 37 to 56.