Two new species of aquatic acentropines, Petrophila anna, new species, and Petrophila cornvillia, new species, are described and illustrated from northcentral Arizona, U.S.A. Both species were initially found in Oak Creek which traverses both Coconino and Yavapai counties, but were observed most frequently at and below Red Rock Crossing in Coconino County where the creek flows southwest to the Verde River. Observations regarding adult flight period, a unique cocoon texture created by sand, and larval mandible morphology are summarized. A table comparing the genitalic differences between the two new species and three externally similar species of Petrophila is provided.