In soil, mites (Acari) are the most abundant and diverse arthropods. They play an important ecological role in maintaining soil health. Among acarines, mesostigmatids are the most abundant group occurring in soil. The present study was undertaken in order to explore the diversity and seasonal occurrence of mesostigmatid mites in three tea gardens maintaining different agro-practices (viz., conventional, organic and biorational) in Assam India. The survey was done over 2 years (August 2016–July 2018). Mesostigmatid mites were extracted by using the heat desiccation method in a Tullgren funnel. The study revealed the occurrence of 41 species of mesostigmatid mites belonging to 17 families and 31 genera in the three tea gardens. Populations of mesostigmatid mites exhibited a fluctuating trend being higher in monsoon (June- September), slightly declining in post monsoon period (October- November), moderate during winter (December- February), and noticeably reduced in summer (March-May). Shannon- diversity index (H′) was found to be highest in the organic tea garden (3.01), followed by biorational tea garden (2.76), and conventional tea garden (2.41).