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1 November 2002 Karyotypes of Human Lymphocytes Exposed to High-Energy Iron Ions
M. Durante, K. George, H. Wu, F. A. Cucinotta
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Durante, M., George, K., Wu, H. and Cucinotta, F. A. Karyotypes of Human Lymphocytes Exposed to High-Energy Iron Ions. Radiat. Res. 158, 581–590 (2002).

Chromosomal aberrations were analyzed using multicolor fluorescence in situ hybridization (mFISH) in human peripheral blood lymphocytes after in vitro exposure to γ rays or accelerated 56Fe ions (1 GeV/nucleon, 145 keV/μm) at Brookhaven National Laboratory (Upton, NY). Doses of 0.3 and 3 Gy were used for both radiation types. Chromosomes were prematurely condensed by a phosphatase inhibitor (calyculin A) to avoid the population selection bias observed at metaphase as a result of the severe cell cycle delays induced by heavy ions. A total of 1053 karyotypes (G2 and M phases) were analyzed in irradiated lymphocytes. Results revealed different distribution patterns for chromosomal aberrations after low- and high-LET radiation exposures: Heavy ions induced a much higher fraction of cells with multiple aberrations, while the majority of the aberrant cells induced by low doses of γ rays contained a single aberration. The high fraction of complex-type exchanges after heavy ions leads to an overestimation of simple-type asymmetrical interchanges (dicentrics) from analysis of Giemsa-stained samples. However, even after a dose of 3 Gy iron ions, about 30% of the cells presented no complex-type exchanges. The involvement of individual chromosomes in exchanges was similar for densely and sparsely ionizing radiation, and no statistically significant evidence of a nonrandom involvement of specific chromosomes was detected.

M. Durante, K. George, H. Wu, and F. A. Cucinotta "Karyotypes of Human Lymphocytes Exposed to High-Energy Iron Ions," Radiation Research 158(5), 581-590, (1 November 2002).[0581:KOHLET]2.0.CO;2
Received: 29 April 2002; Accepted: 1 June 2002; Published: 1 November 2002
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