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1 October 2005 Cellular Monitoring of the Nuclear Factor κB Pathway for Assessment of Space Environmental Radiation
Christa Baumstark-Khan, Christine E. Hellweg, Andrea Arenz, Matthias M. Meier
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Abstract

Baumstark-Khan, C., Hellweg, C. E., Arenz, A. and Meier, M. M. Cellular Monitoring of the Nuclear Factor κB Pathway for Assessment of Space Environmental Radiation. Radiat. Res. 164, 527–530 (2005).

A screening assay for the detection of NF-κB-dependent gene induction using the destabilized variant of the reporter protein enhanced green fluorescent protein (d2EGFP) is used for assessing the biological effects of accelerated heavy ions as a model of space environmental radiation conditions. The time course of d2EGFP expression and therefore of activation of NF-κB-dependent gene expression was measured after treatment with TNFA or after heavy-ion exposure using flow cytometry. The reported experiments clearly show that accelerated argon ions (95 MeV/nucleon, LET 230 keV/μm) induce the NF-κB pathway at low particle densities (1–2 particle hits per nucleus), which result in as few as 5–50 induced DSBs per cell.

Christa Baumstark-Khan, Christine E. Hellweg, Andrea Arenz, and Matthias M. Meier "Cellular Monitoring of the Nuclear Factor κB Pathway for Assessment of Space Environmental Radiation," Radiation Research 164(4), 527-530, (1 October 2005). https://doi.org/10.1667/RR3397.1
Received: 28 July 2004; Accepted: 1 December 2004; Published: 1 October 2005
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