Translator Disclaimer
1 January 2006 Acute Effects of Iron-Particle Radiation on Immunity. Part II: Leukocyte Activation, Cytokines and Adhesion
Daila S. Gridley, Radha Dutta-Roy, Melba L. Andres, Gregory A. Nelson, Michael J. Pecaut
Author Affiliations +
Abstract

Gridley, D. S., Dutta-Roy, R., Andres, M. L., Nelson, G. A. and Pecaut, M. J. Acute Effects of Iron-Particle Radiation on Immunity. Part II: Leukocyte Activation, Cytokines and Adhesion. Radiat. Res. 165, 78–87 (2006).

The effects of high-linear energy transfer (LET) radiation on immune function have not been clearly established. The major goal of this study was to evaluate leukocyte responses after whole-body exposure to high-LET radiation. C57BL/6 mice were exposed to 0, 0.5, 2 and 3 Gy 56Fe26 particles (1055 MeV/nucleon, 148.2 keV/μm) and killed humanely 4 days after exposure. Spontaneous synthesis of DNA in blood and spleen cells was increased significantly in groups receiving either 2 or 3 Gy (P < 0.001). In contrast, a significant depression in the response of T lymphocytes to phytohemagglutinin (PHA) and concanavalin A (ConA) was noted (P < 0.005); the response to lipopolysaccharide (LPS), a B-cell mitogen, was similar among groups. A cytometric bead array assay revealed that the level of tumor necrosis factor α (Tnfa) secreted by splenocytes increased significantly with increasing 56Fe-particle dose (P < 0.05); interferon γ, interleukin2 (Il2), Il4 and Il5 were unaffected. Flow cytometry analysis showed that 2 and 3 Gy markedly reduced splenic mononuclear cells expressing the activation markers CD25 and CD71, both with and without the T-cell marker CD3 (P < 0.05); proportions also varied significantly. Similar patterns were noted in mononuclear and granular cells with adhesion markers CD11b and, to a lesser extent, CD54 (P < 0.05). The results show that a single, acute exposure to high-LET radiation induced changes that can profoundly alter leukocyte functions. The implications of the data are discussed in relation to low-LET radiation, altered gravity, and space flight.

Daila S. Gridley, Radha Dutta-Roy, Melba L. Andres, Gregory A. Nelson, and Michael J. Pecaut "Acute Effects of Iron-Particle Radiation on Immunity. Part II: Leukocyte Activation, Cytokines and Adhesion," Radiation Research 165(1), 78-87, (1 January 2006). https://doi.org/10.1667/RR3490.1
Received: 24 January 2005; Accepted: 1 September 2005; Published: 1 January 2006
JOURNAL ARTICLE
10 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top