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1 October 2006 Association of Ionizing Radiation-Induced Foci of NBS1 with Chromosomal Instability and Breast Cancer Susceptibility
Masanori Someya, Koh-ichi Sakata, Hiroshi Tauchi, Yoshihisa Matsumoto, Asako Nakamura, Kenshi Komatsu, Masato Hareyama
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Abstract

Someya, M., Sakata, K., Tauchi, H., Matsumoto, Y., Nakamura, A., Komatsu, K. and Hareyama, M. Association of Ionizing Radiation-Induced Foci of NBS1 with Chromosomal Instability and Breast Cancer Susceptibility. Radiat. Res. 166, 575–582 (2006).

NBS1, a protein essential for DNA double-strand break repair, relocalizes into subnuclear structures upon induction of DNA damage by ionizing radiation, forming ionizing radiation-induced foci. We compared radiation-induced NBS1 foci in peripheral blood lymphocytes (PBLs) from 46 sporadic breast cancer patients and 30 healthy cancer-free volunteers. The number of persistent radiation-induced NBS1 foci per nucleus at 24 h after irradiation for patients with invasive cancer was significantly higher than for normal healthy volunteers. The frequency of spontaneous chromosome aberration increased as the number of persistent radiation-induced NBS1 foci increased, indicating that the number of persistent radiation-induced NBS1 foci might be associated with chromosome instability. There was also an inverse correlation between the number of radiation-induced NBS1 foci and the activity of DNA-dependent protein kinase (DNA-PK), which plays an important role in the nonhomologous end-joining (NHEJ) pathway, another mechanism of DNA DSB repair, indicating a close interrelationship between homologous recombination (HR) and NHEJ in DNA DSB repair. In conclusion, the number of persistent radiation-induced NBS1 foci is associated with chromosomal instability and risk of sporadic breast cancer and hence might be used to select individuals for whom a detailed examination is necessary because of their increased susceptibility to breast cancer, although refinement of the techniques for technical simplicity and accuracy will be required for clinical use.

Masanori Someya, Koh-ichi Sakata, Hiroshi Tauchi, Yoshihisa Matsumoto, Asako Nakamura, Kenshi Komatsu, and Masato Hareyama "Association of Ionizing Radiation-Induced Foci of NBS1 with Chromosomal Instability and Breast Cancer Susceptibility," Radiation Research 166(4), 575-582, (1 October 2006). https://doi.org/10.1667/RR0638.1
Received: 23 March 2006; Accepted: 1 June 2006; Published: 1 October 2006
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