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1 June 2007 Effect of Treatment Schedule on the Interaction of Cisplatin and Radiation in Human Lung Cancer Cells
Yoshiko Asaka-Amano, Yuichi Takiguchi, Mari Yatomi, Katsushi Kurosu, Yasunori Kasahara, Nobuhiro Tanabe, Koichiro Tatsumi, Takayuki Kuriyama
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Abstract

Asaka-Amano, Y., Takiguchi, Y., Yatomi, M., Kurosu, K., Kasahara, Y., Tanabe, N., Tatsumi, K. and Kuriyama, T. Effect of Treatment Schedule on the Interaction of Cisplatin and Radiation in Human Lung Cancer Cells. Radiat. Res. 167, 637–644 (2007).

This study was designed to determine the effects of the treatment schedule on the interaction between cisplatin and radiation. Cells of a human squamous cell lung cancer cell line were treated with cisplatin and radiation using three treatment protocols: 1-h exposure to cisplatin immediately followed by irradiation (A), 4-day continuous exposure to cisplatin immediately followed by irradiation (B), and 1-h exposure to cisplatin followed by irradiation after a 4-day interval (C). The interactions were assessed by isobologram, cell cycle distribution and apoptosis. The combination resulted in a additive effect in every protocol. Cell cycle accumulation at G2/M phase before irradiation was observed in Protocols B and C, whereas no cell cycle shift in the limited time course was noted in Protocol A. Although a 4-day continuous exposure to cisplatin and a 1-h exposure to cisplatin followed by a 4-day interval before irradiation caused significantly increased apoptosis, an additional increase in apoptosis after irradiation was not observed in Protocols B and C, whereas Protocol A showed an additional increase. Despite a cell cycle shift favoring radiation sensitivity, the drug–radiation interactions in Protocols B and C were additive, possibly because of negative effects including induction of a durable G2/M-phase arrest and suppression of apoptosis by cisplatin.

Yoshiko Asaka-Amano, Yuichi Takiguchi, Mari Yatomi, Katsushi Kurosu, Yasunori Kasahara, Nobuhiro Tanabe, Koichiro Tatsumi, and Takayuki Kuriyama "Effect of Treatment Schedule on the Interaction of Cisplatin and Radiation in Human Lung Cancer Cells," Radiation Research 167(6), 637-644, (1 June 2007). https://doi.org/10.1667/RR0739.1
Received: 28 June 2006; Accepted: 1 January 2007; Published: 1 June 2007
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