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16 December 2011 l-Arginine is a Radioprotector for Hematopoietic Progenitor Cells
Linda L Pearce, Xichen Zheng, Sandra Martinez-Bosch, Patrick P Kerr, Pornsri Khlangwiset, Michael W Epperly, Mitchell P Fink, Joel S Greenberger, Jim Peterson
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Abstract

l-Arginine is shown to protect hematopoietic progenitor (32D cl 3) cells from death due to exposure to γ radiation (137Cs). Some of the other intermediates in the urea cycle, namely ornithine and citrulline, plus urea itself, were not found to have any significant impact on cell survival after irradiation. Intriguingly, supplementation of irradiated cells with l-arginine results in decreased production of peroxynitrite, suggesting that suppression of superoxide generation by nitric oxide synthase in one or more microenvironments is an important factor in the observed radioprotection. The absence of any radioprotective effect of l-arginine in cells at 3% oxygen also confirms the involvement of one or more oxygen-derived species. Knockdown experiments with nitric oxide synthase (NOS) siRNAs in cells and NOS knockout animals confirm that the observed radioprotection is associated with nNOS (NOS-1). l-Arginine also ameliorates the transient inhibition of the electron-transport chain complex I that occurs within 30 min of completing the dose (10 Gy) and that appears to be a functional marker for postirradiation mitochondrial oxidant production.

Linda L Pearce, Xichen Zheng, Sandra Martinez-Bosch, Patrick P Kerr, Pornsri Khlangwiset, Michael W Epperly, Mitchell P Fink, Joel S Greenberger, and Jim Peterson "l-Arginine is a Radioprotector for Hematopoietic Progenitor Cells," Radiation Research 177(6), 792-803, (16 December 2011). https://doi.org/10.1667/RR1281.1
Received: 16 November 2007; Accepted: 1 November 2011; Published: 16 December 2011
JOURNAL ARTICLE
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