Translator Disclaimer
6 May 2013 Development of Serum Iron as a Biological Dosimeter in Mice
Xiao-hong Zhang, Zhi-chao Lou, Ai-lian Wang, Xiao-dan Hu, Hai-qian Zhang
Author Affiliations +
Abstract

Even though serum iron is a commonly used parameter in iron metabolism, it has not yet been applied for biological dosimetry purpose. A new biological dosimeter based on serum iron has been developed in this work. Serum iron levels in mice subjected to gamma rays from a 60Co source were detected with the use of ferrous. The doses are from 0.2–7 Gy with a dose rate of 0.2 Gy/min. The results demonstrate that serum iron level increases with increasing dose. The detection limit based on serum iron has a lower limit of dose detection of about 0.5 Gy and the maximal increase of serum iron observed is maintained 4 h after γ irradiation. Therefore the best suggested time for blood collection is within 4 h after γ irradiation. Two dose-response relationships were observed with both according to degrees of the increase of serum iron levels and different intervals after γ irradiation. The first is a linear relationship of y = 0.98x 6.76 (r = 0.98) obtained 10 min after γ irradiation; the second is the linear quadratic relationship of y = −0.07x2 1.02x 6.45 (r = 0.99) obtained 7 days after γ irradiation. The absorbed doses of mice estimated with the use of both these two dose-response relationships were close to the actual dose of 1 Gy. It is concluded that serum iron is a quick, simple and sensitive biomarker for early assessment of the absorbed dose of mice.

Xiao-hong Zhang, Zhi-chao Lou, Ai-lian Wang, Xiao-dan Hu, and Hai-qian Zhang "Development of Serum Iron as a Biological Dosimeter in Mice," Radiation Research 179(6), 684-689, (6 May 2013). https://doi.org/10.1667/RR3142.1
Received: 29 July 2012; Accepted: 1 January 2013; Published: 6 May 2013
JOURNAL ARTICLE
6 PAGES


Share
SHARE
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top