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3 May 2013 Functional Proteomics Analysis to Study ATM Dependent Signaling in Response to Ionizing Radiation
Amrita K. Cheema, Rency S. Varghese, Olga Timofeeva, Lihua Zhang, Alexander Kirilyuk, Fereshteh Zandkarimi, Prabhjit Kaur, Habtom W. Ressom, Mira Jung, Anatoly Dritschilo
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Abstract

Ataxia telangiectasia (AT) is a human genetic disease characterized by radiation sensitivity, impaired neuronal development and predisposition to cancer. Using a genetically defined model cell system consisting of cells expressing a kinase dead or a kinase proficient ATM gene product, we previously reported systemic alterations in major metabolic pathways that translate at the gene expression, protein and small molecule metabolite levels. Here, we report ionizing radiation induced stress response signaling arising from perturbations in the ATM gene, by employing a functional proteomics approach. Functional pathway analysis shows robust translational and post-translational responses under ATM proficient conditions, which include enrichment of proteins in the Ephrin receptor and axonal guidance signaling pathways. These molecular networks offer a hypothesis generating function for further investigations of cellular stress responses.

Amrita K. Cheema, Rency S. Varghese, Olga Timofeeva, Lihua Zhang, Alexander Kirilyuk, Fereshteh Zandkarimi, Prabhjit Kaur, Habtom W. Ressom, Mira Jung, and Anatoly Dritschilo "Functional Proteomics Analysis to Study ATM Dependent Signaling in Response to Ionizing Radiation," Radiation Research 179(6), 674-683, (3 May 2013). https://doi.org/10.1667/RR3198.1
Received: 19 September 2012; Accepted: 1 January 2013; Published: 3 May 2013
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