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29 April 2013 Ionizing Radiation Leads to the Replacement and de novo Production of Colonic Lgr5 Stem Cells
Kensuke Otsuka, Nobuyuki Hamada, Junji Magae, Hideki Matsumoto, Yuko Hoshi, Toshiyasu Iwasaki
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Abstract

Tissue stem cells have self-renewal capability throughout their whole life, which is high enough to lead to the accumulation of DNA damage in a stem cell pool. Whether radiation-induced damage accumulates in tissue stem cells remains unknown, but could be investigated if the fate of tissue stem cells could be followed after irradiation. To realize this goal, we used an Lgr5-dependent lineage tracing system that allows the conditional in vivo labeling of Lgr5 intestinal stem cells and their progeny. We found that radiation induced loss of Lgr5 stem cells in the colon, but not in the duodenum. Interestingly, the loss of colonic Lgr5 cells was compensated by de novo production of Lgr5 cells, which increased after irradiation. These findings show that ionizing radiation effectively stimulates the turnover of colonic Lgr5 stem cells, implying that radiation-induced damage does not accumulate in the colonic Lgr5 stem cells by this mechanism.

Kensuke Otsuka, Nobuyuki Hamada, Junji Magae, Hideki Matsumoto, Yuko Hoshi, and Toshiyasu Iwasaki "Ionizing Radiation Leads to the Replacement and de novo Production of Colonic Lgr5 Stem Cells," Radiation Research 179(6), 637-646, (29 April 2013). https://doi.org/10.1667/RR3253.1
Received: 9 November 2012; Accepted: 1 February 2013; Published: 29 April 2013
JOURNAL ARTICLE
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