Translator Disclaimer
4 April 2014 Functional Modification of Drosophila Intestinal Stem Cells by Ionizing Radiation
Jung-Hoon Pyo, Joung-Sun Park, Hyun-Jin Na, Ho-Jun Jeon, Shin-Hae Lee, Joong-Gook Kim, So-Young Park, Young-Woo Jin, Young-Shin Kim, Mi-Ae Yoo
Author Affiliations +
Abstract

Although the diverse effects of ionizing radiation on biological and pathological processes at various levels ranging from molecular to whole body are well studied, the effects on adult stem cells by ionizing radiation remain largely unknown. In this study, we characterized the functional modifications of adult Drosophila midgut intestinal stem cells after ionizing radiation treatment. A dose of 10 Gy of radiation decreased the proliferative capacity of intestinal stem cells. Interestingly, after irradiation at 2 Gy, the intestinal stem cells exhibited increased proliferative activity, misdifferentiation and γH2AvD and 8-oxo-dG levels. In addition, the guts irradiated with 2 Gy showed increased JNK and AKT activities. Furthermore, we showed that 2 Gy of ionizing radiation induced centrosome amplification in intestinal stem cells of adult midguts. Our data gives molecular insights into the effects of ionizing radiation on functional modifications of stem cells. The adult Drosophila midgut intestinal stem cells offer a potentially rich new system for the exploration of the biological effects of ionizing radiation.

Jung-Hoon Pyo, Joung-Sun Park, Hyun-Jin Na, Ho-Jun Jeon, Shin-Hae Lee, Joong-Gook Kim, So-Young Park, Young-Woo Jin, Young-Shin Kim, and Mi-Ae Yoo "Functional Modification of Drosophila Intestinal Stem Cells by Ionizing Radiation," Radiation Research 181(4), 376-386, (4 April 2014). https://doi.org/10.1667/RR13545.1
Received: 7 September 2013; Accepted: 1 January 2014; Published: 4 April 2014
JOURNAL ARTICLE
11 PAGES


Share
SHARE
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top