Secondary Structure Alterations of Histones H2A and H2B in X-Irradiated Human Cancer Cells: Altered Histones Persist in Cells for at Least 24 Hours

Yudai Izumi; Satoshi Yamamoto; Kentaro Fujii; Akinari Yokoya

doi:10.1667/RR14071.1

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Yudai Izumi,^1,*,** Satoshi Yamamoto,^2,*** Kentaro Fujii,^1,** Akinari Yokoya^1,3,**

¹a Advanced Science Research Center, Japan Atomic Energy Agency (JAEA), Japan
²b College of Science, Ibaraki University, Japan
³c Graduate School of Science and Engineering, Ibaraki University, Japan

^*Address for correspondence: Quantum Beam Science Directorate, Japan Atomic Energy Agency, 2-4 Ooaza-shirakata, Tokai-mura, Naka-gun, Ibaraki 319-1195, Japan; email: izumi.yudai@jaea.go.jp and yudaiizumi@gmail.com.
^**Current affiliation: Quantum Beam Science Directorate, Japan Atomic Energy Agency (JAEA), Japan.
^***Current affiliation: Graduate School of Science and Engineering, Ibaraki University, Japan.

Abstract

We measured and compared the circular dichroism (CD) spectra and secondary structures of histone proteins H2A, H2B and their variants extracted from X-irradiated and unirradiated human HeLa cells. Compared to unirradiated cells, a relative increase in α-helix structure and decrease in other secondary structures was observed in X-irradiated cells. These structural alterations persisted for at least 24 h, which is substantially longer than the 2 h generally known to be required for DNA double-strand break repair.

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Yudai Izumi, Satoshi Yamamoto, Kentaro Fujii, and Akinari Yokoya "Secondary Structure Alterations of Histones H2A and H2B in X-Irradiated Human Cancer Cells: Altered Histones Persist in Cells for at Least 24 Hours," Radiation Research 184(5), 554-558, (1 November 2015). https://doi.org/10.1667/RR14071.1

Received: 2 March 2015; Accepted: 1 August 2015; Published: 1 November 2015

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