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16 October 2018 Performance of Fluorescent Cell-Labeling Dyes under Simulated Europa Mission Radiation Doses
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Abstract

We investigated the performance of several commonly used fluorescent dyes after exposure to a simulated Europa mission total ionizing radiation dose of 300 krad (3 kGy) applied using a 60Co source. Dyes irradiated in aqueous solution or as lyophilized powders were evaluated for absorbance and emission spectra, quantum yield, and where appropriate, ability to label cells or nucleic acids. Although some dyes showed significant increase or decrease in quantum yield with the dose, their spectra and cell-labeling properties remained essentially unchanged after irradiation in powder form. Irradiation in aqueous solution led to significantly greater changes, including a large blue shift in the DNA intercalator propidium iodide. These results suggest that many fluorescent probes are appropriate for use in astrobiological missions to Europa, but that SYTO9 and propidium iodide should be used with caution or not mixed with each other, as is commonly done in “Live/Dead” labeling applications.

©2018 by Radiation Research Society
Iulia Hanczarek, Aaron J. Kenna, Chris Lindensmith, and Jay Nadeau "Performance of Fluorescent Cell-Labeling Dyes under Simulated Europa Mission Radiation Doses," Radiation Research 190(6), 634-644, (16 October 2018). https://doi.org/10.1667/RR15187.1
Received: 23 July 2018; Accepted: 10 September 2018; Published: 16 October 2018
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