Translator Disclaimer
24 January 2022 A Combination of Iohexol Treatment and Ionizing Radiation Exposure Enhances Kidney Injury in Contrast-Induced Nephropathy by Increasing DNA Damage
Shu Fujino, Jying Sun, Shinya Nakayama, Yasunori Horikoshi, Yasuha Kinugasa, Mari Ishida, Chiemi Sakai, Takeshi Ike, Shigehiro Doi, Takao Masaki, Satoshi Tashiro
Author Affiliations +
Abstract

Contrast media has been shown to induce nephropathy (i.e., contrast-induced nephropathy) after various types of radiological examinations. The molecular mechanism of contrast-induced nephropathy has been unclear. In this study, we investigated the mechanism of contrast-induced nephropathy by examining the effects of combined treatment of contrast medium and ionizing radiation on kidney cells in vitro and kidney tissue in vivo. In human renal tubular epithelium cells, immunofluorescence analysis revealed that iohexol increased the numbers of radiation-induced γH2AX nuclear foci. The numbers of γH2AX nuclear foci remained high at 24 h, suggesting that some radiation-induced double-strand breaks remain unrepaired in the presence of iohexol. We established a mouse model of contrast-induced nephropathy, then showed that iohexol and ionizing radiation synergistically reduced renal function and induced double-strand breaks. Importantly, iohexol induced significant macrophage accumulation and oxidative DNA damage in the kidneys of contrast-induced nephropathy model mice in the absence of ionizing radiation; these effects were amplified by ionizing radiation. The results suggest that underlying inflammation and oxidative DNA damage caused by iohexol contribute to the enhancement of radiation-induced double-strand breaks, leading to contrast-induced nephropathy.

©2022 by Radiation Research Society. All rights of reproduction in any form reserved.
Shu Fujino, Jying Sun, Shinya Nakayama, Yasunori Horikoshi, Yasuha Kinugasa, Mari Ishida, Chiemi Sakai, Takeshi Ike, Shigehiro Doi, Takao Masaki, and Satoshi Tashiro "A Combination of Iohexol Treatment and Ionizing Radiation Exposure Enhances Kidney Injury in Contrast-Induced Nephropathy by Increasing DNA Damage," Radiation Research 197(4), 384-395, (24 January 2022). https://doi.org/10.1667/RADE-21-00178.1
Received: 7 September 2021; Accepted: 10 December 2021; Published: 24 January 2022
JOURNAL ARTICLE
12 PAGES


Share
SHARE
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top