A new species of Glassfrog of the genus Nymphargus from the Andean cloud forest of Cordillera Oriental, Colombia, is described. This species can be recognized by some morphological characteristics that include: parietal peritoneum 1/2–3/4 white, visceral and hepatic peritonea translucent; webbing on hand reduced between outer fingers; and males lacking humeral spine. The new species reported herein is close related with Nymphargus garciae of the Central Andes and is added to the 20 previously known species of the family that occur in the eastern Andes of Colombia. Moreover, it is the northernmost record for the genus in the Cordillera Oriental.