Intraspecific morphological variation has been cited as the main difficulty in species delimitation in the Dendropsophus marmoratus species group. However, acoustic characters of the advertisement calls seem to be helpful to establish limits between species of the group. When the advertisement call of D. nahdereri was described, questions were raised as to some traits that might be shared with other members of the D. marmoratus group. Although six of the eight species recognized for the group have had their advertisement calls described, some of these require revisions due to certain inconsistencies in the original descriptions. Herein the advertisement call of D. seniculus is redescribed on the basis of analysis of 143 calls recorded from seven individuals in the Municipality of Silva Jardim, Rio de Janeiro state, Brazil. A physiologically based note definition is used owing to its advantages in recognizing primary homology. Among the novelties reported for the group are: a change in the dominant frequency within the call, the presence of more than two harmonics, and a long, frequency modulated, final pulse. The original description of the call was based on an audiospectrogram with side-bands that was mistakenly interpreted as having too many harmonics and no pulses. Acoustic differences and similarities among the species reinforce the use of this character source in the taxonomy and phylogeny of the group. Because D. seniculus is acoustically more similar to D. soaresi than to the other species in the group, the phylogenetic relevance of this similarity should be tested.