We describe the advertisement call of the “tink” frog Diasporus anthrax, an endemic species to the low-mid elevations from the Magdalena river valley in northern Colombia. We also conducted a comparative analysis including data from other species within the genus (D. diastema, D. ventrimaculatus, and D. citrinobapheaus). The advertisement call D. anthrax presents the highest dominant frequency with a shorter duration among all the published Diasporus calls. Diasporus anthrax is rare in museum collections, indicating low detectability in field visual surveys. In contrast, advertisement call surveys suggest high abundance in forest fragments along the Magdalena river valley. Therefore, this species represents a good model to establish bioacoustic monitoring strategies to detect population trends in these highly disturbed ecosystems.
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