We describe the diet composition of Tursiops truncatus (Bottlenose Dolphin) from South Carolina waters. Stomach contents of 136 dolphins stranded dead between 2000 and 2006 were examined. Eighty-two dolphin stomachs contained food items and formed the basis for this study. The emphasis of this study was to compare the stomach contents of dolphins that bore evidence of human interaction but were otherwise healthy with those that appeared to die of natural causes. Forty-two prey species representing 24 families were identified. Dolphins fed predominantly on smaller-sized benthic and demersal fish species. Diets were primarily comprised of members of the family Sciaenidae, with Stellifer lanceolatus (Star Drum) being the most abundant species quantitatively. Lolliguncula brevis (Brief Squid) was the most frequently observed prey item. Overall, dolphins that appeared to have died from natural causes consumed similar species of fish and squid to those that exhibited signs of human interaction. This study represents the first quantitative analysis of prey species comprising the diet of Bottlenose Dolphins found in South Carolina waters.