Chrosomus sp. cf. saylori (Clinch Dace) is an undescribed species that is recognized at the state and federal level as a species in need of conservation. The reproductive biology of Clinch Dace is unknown. Here we use in situ breeding observations to infer the timing and mode of reproduction and laboratory analyses to quantify primary and secondary sex characteristics. We conclude that Clinch Dace spawn from May to July using a nest association. Clinch Dace reach reproductive maturity at 2 years, and have a lower number of mature eggs per female and gonadosomatic index in comparison to other Chrosomus species. There was a 3:1 female-biased sex ratio, and pectoral fin length was the only sexually dimorphic external trait. Low reproductive potential coupled with small population sizes and a fragmented distribution places Clinch Dace at a high risk of extirpation.