Host-fish requirements are unknown for many freshwater mussels, which impedes conservation efforts. We conducted host-fish trials for 2 mussels, Lampsilis straminea (Southern Fatmucket) and Quadrula succissa (Purple Pigtoe). Our objectives were to verify techniques by successfully transforming glochidia from a long-term—brooding mussel with known hosts (L. straminea) and to identify hosts for a short-term—brooding mussel with no confirmed hosts (Q. succissa). Results of the trials confirmed known hosts for L. straminea, identified 4 new hosts for L. straminea, and identified 3 ictalurid hosts for Q. succissa. Based on results of this research, additional host trials are needed for Q. succissa and should focus on ictalurids. This study provided information necessary for the management and conservation of mussels with previously unknown hosts in southeast Alabama and west Florida Gulf Coast drainages, while confirming available resources and techniques used by the Florida Fish and Wildlife Conservation Commission for future host-fish trials.