Myotis septentrionalis (Northern Long-eared Bat) is widely distributed in North America, from the Appalachians to the Maritime Provinces and west to British Columbia. In November 2016, we captured 2 non-reproductive Northern Long-eared Bats, 1 female and 1 male, in nets set in Beaufort County, coastal South Carolina. Previous records for the species indicate its nearest breeding colonies are >350 km away, and it had not been documented in South Carolina outside the Blue Ridge region. Although the Northern Long-eared Bat is known to move between summer roosts and winter hibernacula, it is not considered a long-distance migrant. Our records potentially represent an unrecognized coastal population rather than stray individuals. Such a population may be important for the survival of the species, which has been severely impacted elsewhere in its range by white-nose syndrome, a disease that is caused by the introduced fungal pathogen, Pseudogymnoascus destructans, which has not yet been recorded in coastal South Carolina.