Due to its rarity, restricted range, and nocturnal habits, direct observations of the endangered Glaucomys sabrinus coloratus (Carolina Northern Flying Squirrel) are uncommon. Although a variety of predators have been documented for Glaucomys sabrinus (Northern Flying Squirrel) in the Pacific Northwest, published reports of predation events on the G.s. coloratus subspecies are lacking. Here we report on a predation incident of a Mustela frenata (Long-tailed Weasel) taking a Carolina Northern Flying Squirrel from an owl nest box.