Pocket gophers (Geomys pinetis), Gopher Tortoises, armadillos, and fire ants were the primary soil disturbance agents in a longleaf pine ecosystem. Pocket gopher mounds were the most abundant soil disturbance and covered the greatest percentage of the study area. The most prominent feature of the pocket gopher soil disturbance regime was a strong peak in mound formation from November to January each year in the three-year study, with the location of mound clusters shifting from year to year. During the three-year study, the area disturbed in 0.25-ha plots ranged from 0.7–1.0% yr⁻¹. Pocket gopher mound formation rates were negatively correlated with air temperature and influenced by soil type in some cases, but mostly unaffected by prescribed fires.