Keowee Reservoir has supported an abundant population of native Micropterus coosae (redeye bass) for over 30 years. Recently, redeye bass abundance in this reservoir declined concomitantly with the establishment of angler-introduced Micropterus punctulatus henshalli (Alabama spotted bass). We suspected declines in redeye bass abundance may be related to their hybridizing with the Alabama spotted bass resulting in offspring that are difficult to differentiate from the Alabama spotted bass. Thus, we collected tissue for genetic analyses from what was thought to be pure redeye bass (Jocassee Reservoir, SC), the original source of Alabama spotted bass (Lake Lanier, GA) stocked in Lake Keowee, and suspected redeye bass x Alabama spotted bass hybrids (Keowee Reservoir, SC) to determine if hybridization might be occurring. These analyses confirmed that hybridization among species of Micropterus had occurred in Keowee Reservoir.