We surveyed 3 populations of Scutellaria montana (Large-flowered Skullcap), a federally threatened mint, in southeastern Tennessee, and measured microsite characteristics between Large-flowered Skullcap present and absent plots in close proximity. Large-flowered Skullcap plots were typically associated with relatively open areas in forests. Further, some woody plants were positively associated with Large-flowered Skullcap (e.g., Calycanthus floridus [Common Sweetshrub], Carya glabra [Pignut Hickory]), while others were negatively associated (e.g., Vaccinium stamineum [Gooseberry], Pinus virginiana [Virginia Pine]). Linear regression revealed that Large-flowered Skullcap density increased with percent horizontal cover of grass (i.e., Poaceae) and decreased with percent vertical cover of vegetation. Our results suggest that suitable Large-flowered Skullcap sites may be characterized by secondary forests with an open understory containing grass.