Myotis septentrionalis (Northern Long-eared Bat) is a small, insectivorous bat found in the eastern United States and Canada. Along the east coast, its range is thought to extend as far south as the Great Dismal Swamp in coastal Virginia. We captured six M. septentrionalis in the northern coastal plain region of North Carolina. Field identification was based on characters of ear and tragus length and confirmed with mitochondrial cytochrome c oxidase subunit I sequences. These captures signify the presence of a resident population of M. septentrionalis in the northern coastal plain of North Carolina. Future work is needed to document range limits and hibernation behavior of this species in the piedmont and coastal plain of North Carolina.