A rapid method was developed and validated for PCR analysis of potato psyllids for Candidatus Liberibacter solanacearum (Lso), the causal agent of zebra chip disease of potatoes, Solanum tuberosum L. The method is also suitable for PCR amplification and high-resolution melting analysis of the cytochrome oxidase I gene of potato psyllids, Bactericera cockerelli (Sulc), which is useful for psyllid population studies. The new method takes less than 30 minutes to complete, requires only common laboratory reagents, uses one inexpensive enzyme and no organic solvents, and no lengthy centrifugation steps are necessary. The method should be suitable for rapid testing of large numbers of potato psyllids for studies on zebra chip disease epidemiology and genetic analysis of psyllid populations.