The location of Scyphophorus acupunctatus in different parts of Agave plants was determined in Oaxaca, Mexico. Field samples during 2006 to 2010 included wild and domesticated plants in which S. acupunctatus was found in all its developmental stages. We found nine Agave species not previously reported as attacked by S. acupunctatus, exhibiting different degrees of effect and damage. These new reports represent approximately 40% new scientific knowledge and point out important aspects of the presence of this insect in these plants used to produce the alcoholic beverage mescal. Plants with 4 and 5 damage level had the greatest number of lesions per plant (28.3 and 39.2), adults/leaves (15.7 and 30.5) and larvae/stem (35 and 45) respectively.