Translator Disclaimer
1 June 2014 Detection of Aedes aegypti Mosquitoes Infected with Dengue Virus as a Complementary Method for Increasing the Sensitivity of Surveillance: Identification of Serotypes 1, 2, and 4 by RT-PCR in Quintana Roo, Mexico
Jorge Méndez-Galván, Rosa M. Sánchez-Casas, Alejandro Gaitan-Burns, Esteban E. Díaz-González, Luis A. Ibarra-Juarez, Carlos E. Medina de la Garza, Marco Dominguez-Galera, Pedro Mis-Ávila, Ildefonso Fernández-Salas
Author Affiliations +
Abstract

Sensitivity of monitoring Aedes aegypti (L.) populations was determined to identify the distribution of dengue virus (DENV) during epidemics in Quintana Roo. From September to November 2012, we used a motorized aspirator to collect 2,144 female Ae. aegypti from 569 homes. These were grouped into 220 to use semi-nested RT-PCR for DENV, and positive groups were analyzed individually. Five groups (2.27%) were positive for DENV. Individual analysis yielded eight groups that tested positive, six with DENV-2, one DENV-1, and one DENV-4. The latter was not reported by the surveillance system that year. The mean number of female mosquitoes per household was 3.77 ± 5.71, and the rate of viral infection of Ae. aegypti was 0.4%. Most infected mosquitoes (49%) were concentrated in 10% of the houses. Monitoring Ae. aegypti infected with DENV has the potential to complement the current system of clinical and entomological surveillance.

Jorge Méndez-Galván, Rosa M. Sánchez-Casas, Alejandro Gaitan-Burns, Esteban E. Díaz-González, Luis A. Ibarra-Juarez, Carlos E. Medina de la Garza, Marco Dominguez-Galera, Pedro Mis-Ávila, and Ildefonso Fernández-Salas "Detection of Aedes aegypti Mosquitoes Infected with Dengue Virus as a Complementary Method for Increasing the Sensitivity of Surveillance: Identification of Serotypes 1, 2, and 4 by RT-PCR in Quintana Roo, Mexico," Southwestern Entomologist 39(2), 307-316, (1 June 2014). https://doi.org/10.3958/059.039.0208
Published: 1 June 2014
JOURNAL ARTICLE
10 PAGES


Share
SHARE
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top