Sugarcane borer (Lepidoptera: Pyralidae) feeds on buds and shoots of young plants and tunnels in stalks, causing yield loss and affecting juice quantity and quality of harvested sugarcane (Saccharum officinarum L.). The objective of this research was to evaluate pest damage and identify borer species at La Chontalpa, Tabasco, Mexico. Samples were collected starting in February during plant growth and before harvest. Stalks were 8.9% infested and 1.9% damaged before harvest based on a damage scale for the pest, and 9.4% of plants had dead growing points. Diatraea saccharalis (Fabricius) was the only borer species found in the region. Infestation by D. saccharalis was low in sugarcane in the area.