The soybean weevil, Rhyssomatus nigerrimus Fahraeus, seriously damages vegetative and reproductive stages of soybean, Glycine max (L.) Merr., in Mexico. The weevil is currently controlled with conventional insecticide, and it is necessary to generate effective, sustainable control measures to reduce the negative impact of this new pest. The fungus Beauveria bassiana (Balsamo) Vuillemin has shown potential for management of some coleopteran pest species. The objective of this study was to assess the virulence of eight strains of B. bassiana against R. nigerrimus under laboratory conditions. Results showed that B. bassiana is a microbial agent that can potentially be incorporated into a program to control R. nigerrimus. All of the B. bassiana strains reduced survival of adult weevils, relative to the check. The most aggressive strains were Bb-Hy, Bb-Rhy, and Bb-13, with LC⁵⁰ of 1.07 x 107, 1.55 x 107, and 1.31 x 1010 conidia per milliliter of suspension, respectively. Lethal time means (LT⁵⁰) were between 7.5 and 14.5 days. We discuss implications of the results obtained with the evaluated strains.