Survival and mobility of American cockroaches, Periplaneta americana L, that originated from a wild population were compared in semi-natural and laboratory cool-temperature conditions. In a non-heated building all American cockroaches died when air temperatures were ≤0°C despite having access to wood mulch substrate that remained above freezing. Under constant temperatures of 8, 9, and 10°C, approximately 40% of cockroaches died within 72 hours. Mobility was defined as the ability of a cockroach to right itself when flipped over. At all tested temperatures the percentage of individuals that became immobile increased with time. Survival and mobility increased with temperature. Data showed that adult American cockroaches were not able to survive several days at ≤10°C, suggesting a potential cultural control method for tropical cockroaches.