A fossil species of Pheidole Westwood, 1839 (Formicidae, Myrmicinae) was described based on an amber inclusion found in the Miocene strata at Chiapas, southwestern Mexico. It was named Pheidole pauchil sp. nov., which is the first described species of the genus in Mexican amber. This broadens our understanding of the diversity of Pheidole in the Miocene in the southernmost part of North America.