Sorghum (Sorghum (L.) Moench) is one of the most important cereal crops in the world and produced for food, feed, and forage. However, several species of insect pests affect sorghum production, especially sugarcane aphid, Melanaphis sacchari (Zehntner), the most important recent pest. The objective of this research was to identify composition of functional groups of chemicals during sorghum-aphid interaction. Eight experimental lines and two commercial sorghum hybrids were evaluated at the vegetative and reproductive stages at 70 and 100 days after sowing. The experimental design was a randomized complete block with three replications divided into natural conditions (non-chemical treatment) or with chemical treatment for pest control. The agronomic variables of plant height, leaf area, stalk diameter, internode number, number of aphids per area, and total soluble solids were evaluated. LES267NCT in natural conditions was less infested by aphids and considered a resistant genotype, surpassing commercial hybrid P82G63. Conversely, LESSONU was very infested. Fourier Transform Infrared (FTIR) analysis revealed several functional groups such as OH in hydrogenated aromatic, aliphatic, and phenolic alcoholic compounds, tannins, and methylene groups in aromatic compounds, lipids, and lignin.