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1 July 1996 RAPD-PCR method for detecting DNA polymorphism in Boophilus microplus (Acari: Ixodidae)
Mingyang Lan, Weihua Sheng, Caoqiun Huang, Xiandi Gu, Ying Jiang, Yugang Zhao, Weixian Lu
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Abstract

Random amplified polymorphic DNA (RAPD) has been recently used in detecting genetic variation in insect populations. Here, we describe the use of RAPD-PCR for the study of tick DNA polymorphism. A primer “Pz” with the sequence of CGGCCCGGTA was found to be able to amplify the genome of Boophilus microplus (Canestrini). The pattern of the bands were reproducibly obtained and consisted of 13 discrete DNA fragments with the size of 1290, 1070, 960, 870, 790, 720, 630, 580, 500, 450, 390, 300 and 220 base pairs. The RAPD-PCR, for its simplicity, rapidity, and ability to reveal variation at the DNA level, is an additional taxonomic tool for the study of ticks.

Mingyang Lan, Weihua Sheng, Caoqiun Huang, Xiandi Gu, Ying Jiang, Yugang Zhao, and Weixian Lu "RAPD-PCR method for detecting DNA polymorphism in Boophilus microplus (Acari: Ixodidae)," Systematic and Applied Acarology 1(1), 11-14, (1 July 1996). https://doi.org/10.11158/saa.1.1.3
Accepted: 18 April 1996; Published: 1 July 1996
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KEYWORDS
Boophilus microplus
RAPD-PCR
ticks
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