We report parasitism by the ixodid ticks Amblyomma mixtum, Amblyomma oblongoguttatum and Amblyomma tapirellum on the lesser capybara or poncho, Hydrochoerus isthmius, in Panama. These data are integrated with earlier Panamanian records of Amblyomma auricularium and Rhipicephalus sanguineus s.l. from H. isthmiusand compared with known records of tick species parasitizing the capybara, Hydrochoerus hydrochaeris, in South America. Our collections do not permit us to determine whether the poncho is a significant host for A. mixtum, A. oblongoguttatum or A. tapirellum; rather, our results should be viewed as contributing to the discussion of potential hosts of these tick species.