Genome—specific DNA from four species of spider mites (Tetranychus urticae, T. truncatus, T. cinnabarinus and Panonychus citri) were determined by RAPD (random amplified polymorphism DNA) technology. We designed four oligonucleotide fragments (MIP1, MIP2, VP1 and VP4) as single primers to amplify genomic DNA of these species. Two primers, MIP1 and VP4, were proven to be useful and generated different banding genome—specific DNA fingerprints from the RAPD products. The pattern of bands showed DNA fragments with the size of 455–172bp. Although the pattens of bands varied using different primers, the conclusions of the relationship between four species were similar: T. urticae and T. cinnabarinus are more closely related to each other than to other species. By comparing and analyzing this information, we could distinguish the spider mites at the molecular level.