The products synthesized by the synganglion of Haemaphysalis longicornis were studied in vitro by tissue incubation, thin-layer-chromatography (TLC) and radiochemical assay. The results suggested that the synganglion produced radioisotope-labelled substances when incubated in vitro following a radiochemical assay. The 3H radioactivity (3572 dpm) of hexane extract contained 2 synganglia which was about 41 times that (87 dpm) of the control without synganglion. TLC and LSS analysis revealed that the synthesized products comprised two components: one co-chromatography with standard JH III (Rf = 0.81) and the other (Rf = 0.43) not co-chromatography with standard JHB3, JH III and MF. Our results suggested that the synganglion may synthesize JH III and an unknown substance in this tick.
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Vol. 2 • No. 1