To date, the feather mite family Ptyssalgidae (Acariformes: Analgoidea) has been known from a single species associated with hummingbirds (Apodiformes: Trochilidae). Here, based on our collecting in Mexico, we describe (i) a new genus and species, Tyrannoptyssalges striatusgen. n., sp. n., from a passerine host Tolmomyias sulphurescens (Passeriformes: Tyrannidae) and (ii) four new species of the genus PtyssalgesAtyeo and Gaud, 1979 from hummingbirds (Apodiformes: Trochilidae): Ptyssalges amaziliaesp. n. from Amazilia rutila (Delattre) (type host), A. candida (Bourcier & Mulsant) and A. yucatanensis (Cabot), P. anthracothoracissp. n. from Anthracothorax prevostii (Lesson, R.), P. atyeoisp. n. from Phaethornis longirostris (Delattre), and P. campylopterisp. n. from Campylopterus curvipennis excellens (Wetmore). In addition, we redescribe Ptyssalges major (Trouessart, 1887), the type species of the genus, based on newly collected material from the type host, Eutoxeres aquila, from Panama. Standard morphological descriptions of all mite species are supplemented by CO1 barcoding sequence data. In the genus Ptyssalges, CO1 K2P interspecific genetic distances were 11.39–11.89%, while distances between the single species of Tyrannoptyssalges and species of the genus Ptyssalges were 16.34–17.87%. New, amended diagnoses for the family Ptyssalgidae and the genus Ptyssalges and a key to all known ptyssalgid species are provided. Preliminary hypotheses on the origin and ancestral host associations of ptyssalgids are briefly discussed.