A new species of water mites from Montenegro and Bulgaria, Atractides anae Pešić sp. nov., is described, combining traditional morphological techniques with the analysis of partial mtDNA COI sequences (DNA-barcodes). Based on our molecular and morphological analysis, the new species is most closely related to the crenobiontic A. pennatus (K. Viets 1920) from which it differs by 6.4 (SD = 1.12)% K2P distance. A dataset including the novel COI sequences of 10 Atractides species from Montenegro and Iran is assembled in order to facilitate further studies on the genus by using molecular tools.
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