Molecular identification of some Haemaphysalis species (Acari: Ixodidae) using mitochondrial and nuclear evidences in parts of Iran

Faezeh Faghihi; Asadollah Hosseini-Chegeni; Hamideh Edalat; Omid Banafshi; Zakkyeh Telmadarraiy; Mohammad Mehdi Sedaghat

doi:10.11158/saa.25.5.3

How to translate text using browser tools

15 May 2020 Molecular identification of some Haemaphysalis species (Acari: Ixodidae) using mitochondrial and nuclear evidences in parts of Iran

Faezeh Faghihi, Asadollah Hosseini-Chegeni, Hamideh Edalat, Omid Banafshi, Zakkyeh Telmadarraiy, Mohammad Mehdi Sedaghat

Author Affiliations +

Systematic and Applied Acarology, 25(5):809-820 (2020). https://doi.org/10.11158/saa.25.5.3

Abstract

Haemaphysalis is the second largest ixodid tick genus. We decided to identify the Iranian Haemaphysalis species with the analysis of mitochondrial (COI & 16S rRNA) and nuclear (ITS2) gene sequences. Finally, 21 PCR products were sequenced, successfully. Phylogenetic trees were analyzed using BEAST software and Bayesian Inference method. Ha. sulcata and Ha. punctata were well differentiated using the 16S rRNA, COI and ITS2 genes. According to 16S rRNA and COI phylogenetic trees Ha. inermis is situated as an ancestral clade.

Citation Download Citation

Faezeh Faghihi, Asadollah Hosseini-Chegeni, Hamideh Edalat, Omid Banafshi, Zakkyeh Telmadarraiy, and Mohammad Mehdi Sedaghat "Molecular identification of some Haemaphysalis species (Acari: Ixodidae) using mitochondrial and nuclear evidences in parts of Iran," Systematic and Applied Acarology 25(5), 809-820, (15 May 2020). https://doi.org/10.11158/saa.25.5.3

Received: 12 December 2019; Accepted: 11 March 2020; Published: 15 May 2020

KEYWORDS/PHRASES

PUBLICATION TITLE:

COLLECTION TITLE:

PUBLICATION YEARS