Tick-borne diseases are on the rise globally; however, information is lacking about tick questing behavior. In this laboratory study, we explored tick preferences for stem type (plastic grass, wooden, and metal), questing height, and head orientation. Using 60 Amblyomma americanum adults over three 72-hour replicates, we determined that 21.7% of ticks quested at any given time and that ticks exhibited a strong preference to quest with their heads oriented downwards, irrespective of stem type. Individual ticks tended to quest on only one stem in this study, and a maximum of three. Nonetheless, ticks appeared to prefer questing on wooden and plastic grass stems over metal stems. We did not find an effect of time of day on tick questing rates. Increased understanding of tick questing behavior can improve vector control efforts.