A new species of Pergalumna (Oribatida, Galumnidae) is described from leaf litter of rain forest in the Espejillos environs near the Bolivian Ambor National Park. Pergalumna espejillosensissp. nov. is similar to Pergalumna nasicaPérez-Íñigo & Baggio, 1980 in the presence of protruding rostrum, three pairs of rounded/oval notogastral porose areas and minute interlamellar seta, and in the absence of dorsosejugal suture, but differs by the smaller body length, setiform bothridial seta and long lamellar seta, and the absence of a median pore. An identification key to known species/subspecies of the genus from the Neotropical region is presented.