Comparative studies on molecular techniques for detecting Rickettsia tsutsugamushi in Leptotrombidium deliense and Mice

Zhiyong Xi; Jiacan Li; Xiaoying Zheng; Xie Yan

doi:10.11158/saa.4.1.3

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1 July 1999 Comparative studies on molecular techniques for detecting Rickettsia tsutsugamushi in Leptotrombidium deliense and Mice

Zhiyong Xi, Jiacan Li, Xiaoying Zheng, Xie Yan

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Systematic and Applied Acarology, 4(1):15-20 (1999). https://doi.org/10.11158/saa.4.1.3

Abstract

In order to establish the most sensitive and specific method in the detection of Rickettsia tsutsugamushi in Leptotrombidium deliense, mice and patients, a comparative study on DNA extraction, PCR, hybridization and PCR-hybridization was conducted. The results showed that the sensitivity of PCR-hybridization was 10 times higher than that of PCR and 1000 times higher than that of hybridization. Quick DNA extraction method could take the place of phenol-chloroform method to prepare DNA template when detecting the rickettsiae in mice and patients' blood by PCR. De-inhibitor quick DNA extraction method could improve the detection rate of rickettsiae from chigger mites.

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Zhiyong Xi, Jiacan Li, Xiaoying Zheng, and Xie Yan "Comparative studies on molecular techniques for detecting Rickettsia tsutsugamushi in Leptotrombidium deliense and Mice," Systematic and Applied Acarology 4(1), 15-20, (1 July 1999). https://doi.org/10.11158/saa.4.1.3

Accepted: 1 March 1999; Published: 1 July 1999

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