Lepanthes queremalensis, a new species of Lepanthes from Valle del Cauca, Colombia, is described and illustrated. It shows a set of mixed characters of two other species: L. sinuosa (the cymbiform shape of the lip appendix) and L. ankistra (the shape of the leaf and the lip blades). A key to the Colombian species of Lepanthes section Lepanthes subsection Lepanthes series Mucronatae is presented. Information about its habitat, ecology and distribution is provided.