A new species of Macrotorus (Monimiaceae) from the Brazilian Atlantic rainforest is here described and illustrated: Macrotorus genuflexus. This species, restricted to the Poço das Antas Biological Reserve (situated in the central region of Rio de Janeiro state, Brazil), is the second in the genus Macrotorus. The new species description is based on morphological and cytogenetic (karyotype and genome size) comparative analyses. We also report a new record of Macrotorus utriculatus for the state of Bahia, confirming a potential distribution modelling prediction, and provide comments for the conservation of both species.